Fig. 5.

5′ deletion analysis of the LTR promoter in human colorectal cancer cell lines. ERV-L LTR nucleotides 1–522 and five sequentially 5′ deleted LTRs were cloned into the pGL3B luciferase reporter vector. Plasmids were transiently transfected into DLD-1 and LoVo cells. Luciferase activities are shown relative to the internal control and then to pGL3B to demonstrate fold activation. Bars represent the mean of four independent transfections ± SEM.