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. 2003 Oct 20;100(22):12883–12888. doi: 10.1073/pnas.2135542100

Fig. 3.

Fig. 3.

Analysis of the virus-neutralizing antibody response in TgH(KL25) and TgH(VI10) mice. (A) Total Ig-neutralizing titers were determined in serum from naïve C57BL/6, TgH(KL25), and TgH(VI10) mice. The detection limit of the assay is indicated by a dashed line. Note the different scales for LCMV and VSV. (B) C57BL/6 and TgH(KL25) mice were infected i.v. with 2 × 106 pfu of LCMV-WE, serum samples were collected at the time points indicated, and total Ig- and IgG-neutralizing titers were determined. The IgG detection limit of the assay is indicated by a dashed line. (C) C57BL/6 and TgH(VI10) mice were infected i.v. with 2 × 106 pfu of VSV-IND. Total Ig- and IgG-neutralizing titers were determined in the serum. (D)(Left) CD4-depleted TgH(KL25) mice were infected with 2 × 106 pfu of LCMV-WE, and virus-neutralizing antibodies were determined from 10-fold prediluted sera. (Right) Neutralizing antibody response elicited in CD4-depleted TgH(VI10) mice after infection with 2 × 106 pfu of VSV. Sera were 40-fold prediluted. In A, data represent the mean ± SD of five mice from one experiment. The data shown in B and C display the mean ± SD of three mice and represent one of three similar experiments. Data shown in D represent the mean ± SD of at least two animals with one of two experiments shown.