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. 2003 Oct 20;100(22):12911–12916. doi: 10.1073/pnas.1735525100

Fig. 2.

Fig. 2.

Biological outcomes of IgE binding to mast cells in the absence of added growth factors. (A) BMCMC were incubated with the indicated IgEs for 24 h before analysis of cellular content of histamine. (B) BMCMC were incubated with the indicated IgEs for 50 min before analysis of histamine released into culture supernatants. (C) Comparison of kinetics of histamine release induced by 5 μg/ml SPE-7 IgE and that induced from H1 DNP-ε-206 IgE-sensitized BMCMC after stimulation (Stim.) with 100 ng/ml DNP21 HSA. (D) Leukotriene release into culture supernatants from BMCMC incubated with the indicated IgEs for 50 min. (E) Internalization of FcεRI. BMCMC were incubated with the indicated concentrations of H1 DNP-ε-206 or SPE-7 IgE for 24 h before analysis of FcεRI expression. (F) DNA synthesis. BMCMC were incubated with the indicated IgEs for 24 h in the presence of [3H]thymidine for the last 6 h. (G) Effects of low-level cross-linking vs. IgE binding. BMCMC were incubated with the indicated IgEs (5 μg/ml) in the presence or absence of 1 or 10 ng/ml DNP21 HSA for 3 days before survival assays.