Fig. 3.
Inhibition of IFN-γ by IL-4 in CD3+ cells is mediated by SOM. FACS of CD3+ cells expressing IFN-γ was performed on primary cells isolated from SOM+/+ (a and b) and SOM-/- (c and d) mouse stomachs. Primary cultures were exposed to PBS, H. felis, IL-4, or OCT. In addition, cultures were also exposed to H. felis plus IL-4 or OCT with or without SOM antagonist (ANT). Results are expressed as the mean ± SEM for n = 6 experiments performed in triplicate incubations. *, P < 0.05 vs. PBS-treated cultures (unpaired t test).