Figure 5.

Colonies of C. albicans cells grown for 5 days at 37°C on solid Spider medium containing mannitol. Wild-type strain SC5314 (13) (A), ura3/ura3 cst20Δ/cst20Δ::URA3 strain CDH22 (B), ura3/ura3 cst20Δ/cst20Δ::CST20::URA3 strain CDH36 (obtained by reintegration of CST20 into strain CDH25 by homologous recombination using linearized plasmid pDH190) (C), ura3/ura3 cst20Δ/cst20Δ strain CDH25 transformed with plasmids pYPB1-ADHpt (D) and pYPB1-ADHpt-HST7 (E), ura3/ura3 hst7Δ/hst7Δ strain CDH12 transformed with plasmids pVEC (F), pVEC-HST7 (G), pYPB1-ADHpt (H), and pYPB1-ADHpt-HST7 (I), and ura3/ura3 cph1/cph1 strain CDH72 [ura3/ura3 derivative of strain JK19 (6)] transformed with pYPB1-ADHpt-HST7 (J). (×2; bar = 2 mm.)