Fig. 2.

TraSH accurately predicts the in vivo growth characteristics of individual mutants. (A) Average TraSH ratios for the indicated genes (in vitro pool/in vivo pool) are plotted on a log scale as a function of time (circles). Individual strains carrying mutations in these genes were mixed with wild-type bacteria and inoculated into mice. The ratio (wild type/mutant) at each time point after infection is plotted (squares) on the same scale as the TraSH data. Data were normalized so the ratio of the inoculum equaled 1. Error bars represent standard deviations. (B) Growth of individual mutants in lung (filled bars) and spleen (open bars). Data were collected as in A from organs harvested after 4 weeks of infection except for Tn::Rv0573 (8 weeks). (C) Attenuated strains do not have in vitro growth defects. Each mutant strain was mixed with wild-type H37Rv and grown in triplicate broth cultures. The ratio of wild type to mutant was determined after inoculation (open bars) and after 10 days of growth (filled bars). Only the ratio for the ΔbioF mutant changed significantly (P = 0.0005; mutant grew faster than wild type).