Table 2. Fusion host range of the HERV-FRD envelope.
Fusion index* |
|||||
---|---|---|---|---|---|
Species | Target cells | HERV-FRD | HERV-W | A-Rless | None |
Mouse | NIH3T3 | 1.0 ± 0.9 | 0.2 ± 0.5 | 41 ± 17 | 0.4 ± 0.3 |
Hamster | CHO | 1.8 ± 0.6 | 1.3 ± 0.8 | 1.8 ± 0.4 | 1.0 ± 0.5 |
Cat | G355—5 | 90 ± 2 | 2.2 ± 3.2 | 83 ± 3 | 0.7 ± 0.8 |
Monkey | Cos-7 | 20 ± 4 | 62 ± 3 | 19 ± 4 | 13 ± 3 |
Human | TE671 | 66 ± 6 | 56 ± 10 | 39 ± 8 | 3.1 ± 1.8 |
293T | 81 ± 7 | 90 ± 4 | 9 ± 3 | 0.3 ± 0.6 | |
HeLa | 1.8 ± 0.7 | 65 ± 9 | 23 ± 6 | 1.0 ± 0.4 |
Target cells were transfected with expression vectors for the HERV-FRD envelope, the HERV-W envelope, the hyperfusogenic mutant amphotropic MLV envelope (A-Rless), or no protein (none) as a negative control, and fusion indices were determined as indicated in Materials and Methods (means ± SDs; n = 5)