Table 3.
Ectopic ch and es organs induced by various ato or sc proteins with mutations in the b domains
| Line | Protein | b domain sequence | No. of organs per wing
|
ch/(ch + es), % | No. of wings | No. of lines | |
|---|---|---|---|---|---|---|---|
| Ectopic ch | Ectopic es | ||||||
| ∗∗∗∗∗∗∗ ∗ | |||||||
| 1 | ato (WT) | KRKRRLAANARERRR | 31.6 ± 4.6 | 31.9 ± 2.2 | 50 | 16 | 5 |
| 2 | sc (WT) | SVQRR---NARERNR | 1.7 ± 0.7 | 57.0 ± 4.3 | 3 | 11 | 3 |
| 3 | sc-m14 | KRKRR---NARERRR | 2.9 ± 0.9 | 21.1 ± 1.0 | 12 | 8 | 4 |
| 4 | sc-m24 | SRQRR---NARERRR | 5.2 ± 1.9 | 24.7 ± 1.1 | 17 | 8 | 4 |
| 5 | sc-m34 | SVQRR---NARERRR | 4.0 ± 1.4 | 23.8 ± 1.3 | 14 | 10 | 4 |
| 6 | sc-m15 | KRKRR---NARERNR | 0.3 ± 0.2 | 20.8 ± 1.7 | 1 | 8 | 4 |
| 7 | sc-m25 | SRQRR---NARERNR | 0.5 ± 0.3 | 18.1 ± 1.9 | 3 | 6 | 2 |
| 8 | sc-in | SVQRRLAANARERNR | 2.1 ± 0.6 | 43.6 ± 2.2 | 5 | 21 | 8 |
| 9 | ato-(R-N) | KRKRRLAANARERNR | 17.1 ± 4.8 | 38.7 ± 1.7 | 31 | 16 | 4 |
| 10 | ato-del | KRKRR---NARERRR | 13.5 ± 2.6 | 31.8 ± 3.2 | 30 | 13 | 2 |
The amino acid sequences of the b domains start at Lys-253 of ato or Ser-100 of sc. WT, wild type. The ato sequence is in boldface type. The conserved residues are marked with ∗. Other columns are described in the legend of Table 1.