Figure 1.

Expression of lacZ mRNA in mouse forebrain. (A) Schematic representation of the DNA constructs used for the generation of transgenic mice. lac-CMK: the 8.5-kbp CaMKIIα promoter region, as well as 84 nucleotides of the 5′ non-coding exon, was fused to the E. coli lacZ gene. The entire 3′-UTR of the CaMKIIα mRNA was placed downstream of the lacZ coding region. lac-A: identical to lac-CMK except that the bovine growth hormone polyadenylylation signal was substituted for the CaMKIIα 3′-UTR. nls-lac-CMK, the tet·O promoter (16) was linked to a modified lacZ gene with an in-phase fusion to the green fluorescent protein (GFP) and a nuclear localization sequence. (B) Northern blot analysis of poly(A)⁺ RNA isolated from the forebrain of the lac-CMK and lac-A mice.