Figure 3.

Analysis of ine transcription in wild-type and ine mutant flies by Northern blot analysis. Total RNA was prepared from adult flies. mRNA was isolated using oligo(dT)-cellulose type 77F (Pharmacia), size-separated on a 1% agarose gel that contained 6% formaldelyde, and then transferred to GeneScreen membrane (DuPont). The 3′ end of the ine cDNA (≈800 bp long) was labeled with [³²P]dCTP and used as probe. Lanes 1–4 are iso bw (the isogenic wild-type parent to ine mutants), ine¹, ine ², and ine ³, respectively. The relative amounts of mRNA loaded in each lane was deduced from a control probe prepared from ribosomal protein L27a (0.75-kb band). Quantitation of L27a band intensity (arbitrary units): iso bw, 1.0; ine¹, 1.5; ine², 1.6; ine³, 2.1. The image was exposed for 3 days and processed with the FujiX BAS 1000 phosphorimager system.