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The effect of gentle perfusion on the shape and magnitude of an astrocyte calcium wave. The + indicates the point of stimulation. (Bar = 100 μm.) (A) Astrocytes participating in a control calcium wave are radially distributed around the stimulation site. (B) The same field of astrocytes participating in a calcium wave during perfusion with positive pressure on the pipette. Participating cells are biased away from the perfusion pipette, and the number of participating cells is reduced.
