Figure 5.

The effect of gentle suction through a pipette close to the stimulation site on the shape and magnitude of astrocytic calcium waves. The + indicates the point of stimulation. (Bar = 100 μm.) (A) Astrocytes participating in a calcium wave in the absence of suction are radially distributed around the stimulation site. (B) The same astrocytes during a calcium wave while negative pressure was applied to the perfusion pipette. The number of participating astrocytes was markedly reduced. (C) Restimulation in the same area with no movement of extracellular fluid showing recovery of the number of participating astrocytes.