Figure 1.

Development of TH-positive cells in mouse trunk neural crest cultures in response to selected growth factors in the TGF-β superfamily. Cultures were grown for 12 days either in medium containing 10% fetal bovine serum and 5% CEE (solid bars) or in medium containing 10% fetal bovine serum (open bars) in the presence of the growth factors indicated. Concentrations of the growth factors were as follows: GDNF, BMP-2, BMP-4, and BMP-6, 10 ng/ml; TGF-β1, TGF-β2, and TGF-β3, 2 ng/ml. The number of TH-positive cells per culture is expressed as the mean ± SEM of three to eight cultures analyzed per condition.