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Dose–response profile of the development of TH-positive cells to GDNF. Neural crest cultures were grown in medium containing 10% fetal bovine serum and 5% CEE with the concentrations of GDNF indicated for 12 days in vitro. They were then processed to reveal TH-positive cells. The number of TH cells is expressed as the mean ± SEM with four cultures analyzed per condition. The 0, 0.1, and 1 ng/ml values are statistically different from the 10 and 50 ng/ml values with P < 0.05.
