Figure 3.

Appearance of mouse trunk neural crest cultures in the presence and absence of GDNF. (A) Fluorescence photograph of TH-positive cells in a cultures grown in CEE medium in the presence of GDNF at 10 ng/ml for 12 days in vitro. Numerous TH-immunoreactive cells bodies and some cellular processes are present. (B) Phase-contrast view of the same field in A, demonstrating the dense packing of the neuron-like cell bodies in these cultures. Many TH-negative cells are intermingled with the TH-positive cells. (C) Fluorescence photograph of a control culture grown in CEE medium in the absence of GDNF. Two TH-positive cells are present. (D) Phase-contrast view of the same field as in C, showing the presence of numerous TH-negative cells with both neuronal and nonneuronal morphologies. (Bar = 30 μm.)