Figure 4.

TH-positive cells that develop in mouse trunk neural crest are also neurofilament-positive. Cultures were prepared as described and grown in medium with 10% fetal bovine serum, 5% CEE, and GDNF (10 ng/ml) for 12 days in vitro. Confocal image of a culture that was double labeled for TH and the middle neurofilament protein after 12 days in vitro. (A) Fluorescent image of TH-positive cells. (B) Fluorescent image of neurofilament-positive cells. Neurofilament-positive processes emanating from other cells in the culture are also observed. (Bar = 5 μm.)