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. 1996 Nov 12;93(23):13274–13279. doi: 10.1073/pnas.93.23.13274

Figure 5.

Figure 5

(A) Time course of development of TH-positive cells in the presence and absence of GDNF. Cultures were grown in the presence (•) or absence (○) of GDNF at 10 ng/ml in medium containing 10% fetal bovine serum and 5% CEE and assayed at the times indicated for number of TH-positive cells. The number of TH-positive cells is expressed as the mean ± SEM with three cultures analyzed per condition. The day 12 GDNF and control values are statistically significant with P < 0.05. (B) Analysis of the temporal requirement for GDNF. Cultures were grown for a total of 12 days in vitro in either the presence (+) or absence (−) of GDNF at 10 ng/ml for the time period indicated. TH-positive cell number per culture is expressed as the mean ± SEM with 12 cultures analyzed per condition. These results indicate the presence of GDNF in the period from 6 to 12 days in vitro is required for a robust increase in TH-positive cell number. The −/− and +/− values are statistically different from the −/+ and the +/+ values with P < 0.05.