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. 2008 Jun;172(6):1529–1541. doi: 10.2353/ajpath.2008.070429

Figure 8.

Figure 8

PI 3-kinase is involved in the Nrf2 pathway during muscle differentiation. A: After 8 hours of treatment with LY294002 or LY303511 in DM, the nuclear extracts were assessed via Western blotting (A, top) and densitometric (A, bottom) analysis. B: After treatment with the indicated doses of LY294002 or LY303511 in DM, the total extracts were assessed via Western blotting analysis. Actin was used as a loading control. C and D: In the two clones (1 and 2) of mock and Δp85 transfectants, nuclear extracts were analyzed via Western blotting analysis to observe Nrf2 translocation in GM or in DM for 8 hours (C). After mock and Δp85 transfectants were cultivated in GM or DM for 24 hours, Western blotting analysis was conducted (D). Mock transfectants were used as negative controls. E and F: Cells were treated for 24 hours with LY294002 or LY303511 in DM and the intracellular (E) and extracellular (F) total GSH content was measured. The data are expressed as the means ± SE of at least three independent experiments. *P < 0.05, versus untreated cells. G: After treatment with 20 μmol/L of SB203580 or PD98059 for 8 (top) or 24 (bottom) hours in DM, the nuclear or total extracts were assessed via Western blotting analysis.