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Western blot analysis of TNF-α and IL-1β effects on IL-6 output by decidual cell monolayers maintained in E2 or E2 + MPA. Confluent, leukocyte-free first trimester decidual cells were incubated for 7 days in 10−8 mol/L E2 or E2 + 10−7 mol/L MPA, then switched to a DM with corresponding steroid(s) ± 1 ng/ml of TNF-α or IL-1β for 24 hours. Conditioned DM was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting (see Materials and Methods).
