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. 2008 Jun;172(6):1580–1590. doi: 10.2353/ajpath.2008.071021

Figure 3.

Figure 3

IGF-II gene expression is derived from P3 in SSc lung fibroblasts. A: RT-PCR of total RNA was performed using primer pairs specific for mRNA species generated from each promoter. As previously described,18 expected PCR product sizes were 363 bp for P1, 249 and 409 bp for P2, 151 bp for P3, and 76 bp for P4. B: IGF-II expression from one representative SSc lung fibroblast shows abundant expression from P3. There is no detectable mRNA from P1, P2, or P4. IGF-II expression from a representative normal lung fibroblast shows no IGF-II expression from any of the four promoters. Adult liver tissue and HepG2 cells served as positive controls for each primer pair. C: Comparison of mRNA from six SSc lung fibroblasts show increased IGF-II expression from P3 compared to six normal lung samples.