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. 2008 Jun;172(6):1580–1590. doi: 10.2353/ajpath.2008.071021

Figure 4.

Figure 4

IGF-II induces collagen type I and fibronectin production in SSc primary lung fibroblasts in a dose-dependent manner (A) compared to normal fibroblasts (B). Equal numbers of fibroblasts were cultured with increasing concentrations of recombinant IGF-II for 48 hours. Collagen (C) and fibronectin (D) expression was quantified using scanning densitometry, and values were normalized to baseline SSc expression levels. SSc fibroblasts show a statistically significant increase in collagen (at 200 ng/ml IGF-II, 3.09 versus 1.0, ±1.8, in arbitrary units; P = 0.016) and fibronectin production (2.39 versus 1.0 ± 1.3; P = 0.019). Normal fibroblasts show peak collagen expression at 50 ng/ml of IGF-II (1.48 versus 0.64 ± 0.64; P = 0.020). Data shown represent experiments using five different SSc and five different normal fibroblasts. Asterisks indicate P < 0.05 compared with no IGF-II stimulation in SSc (one asterisk) and normal fibroblasts (two asterisks).