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. 2008 Jun;172(6):1580–1590. doi: 10.2353/ajpath.2008.071021

Figure 8.

Figure 8

IGF-II-induced ECM is inhibited by PI-3 kinase and JNK inhibitors. Primary lung fibroblasts from SSc (lanes 1 to 6) and normal lungs (lanes 7 to 12) were incubated with 10 μmol/L LY294002 (LY), U0126 (U), JNK inhibitor II (J), or vehicle control (veh) for 1 hour before the addition of IGF-II (200 ng/ml). Fibroblasts were stimulated with IGF-II for 48 hours before collection of lysates and supernatants. IGF-II induced collagen and fibronectin production (lanes 2, 3, 8, 9). This effect is blocked with the addition of a PI-3 kinase inhibitor (lanes 4 and 10) and JNK inhibitor (lanes 6 and 12). MEK inhibition did not have any effect on IGF-II-induced ECM production (lanes 5 and 11).