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. 2008 Jun;172(6):1617–1624. doi: 10.2353/ajpath.2008.070971

Figure 5.

Figure 5

Effect of meth on endogenous IFN-α mRNA (A) and protein (B) expression in macrophages. A: Seven-day-cultured macrophages were treated with or without 100 μmol/L meth for the indicated time points, and then cellular RNA were subjected to the real-time RT-PCR for IFN-α mRNA. B: Cell lysates from seven-day-cultured macrophages treated with or without 100 μmol/L METH for 24 hours were subjected to enzyme-linked immunosorbent assay for IFN-α protein. Data are expressed as IFN-α mRNA (A) or protein (B) levels in meth-treated cells (percentage of control) to those in untreated cells, which are defined as 100%. The results represent the mean ± SD of three independent experiments. Statistical analysis was performed by Student’s t-test and significance is shown with *P < 0.05 and **P < 0.01 (meth versus control).