Skip to main content
. 2008 Jun;172(6):1693–1703. doi: 10.2353/ajpath.2008.080024

Figure 3.

Figure 3

SDF-1 plays a crucial role for the retention of BM-derived cells in the degenerating retina and inhibition of SDF-1 accelerates retinal degeneration. A: Real-time PCR analysis for SDF-1 in the retina of WT mice and rd1/rd1 mice (n = 6 per group). B and C: Retinal flatmount of saline-injected eye and anti-SDF-1 neutralization antibody-injected eye (injected at P8) 15 days after GFP donor BMT. D: Marked reduction of BM-derived cells in the anti-SDF-1-treated eyes (n = 8). E–H: Deep vasculature (P25) of saline- and anti-SDF-1-injected eyes in WT (E, F) and rd10 mice (G, H). No change in anti-SDF-1-injected eyes of WT mice (F), in contrast, loss of microvasculature occurred in anti-SDF-1-injected eyes of rd10 mice (H), compared to saline-injected eyes (G). I–L: Sectional images (P25) of saline- and anti-SDF-1-injected eyes in WT (I, J) and rd10 mice (K, L). No change was observed in WT mice (I, J); however, there was a marked loss of photoreceptor cells in the outer nuclear layer (ONL) in anti-SDF-1-injected eyes of rd10 mice (K, L). GCL, ganglion cell layer; INL, inner nuclear layer. M: Vascular length of deep vasculature (P25) was reduced in anti-SDF-1-injected eyes of rd10 mice (n = 8). N: Number of photoreceptor cells in ONL (P25) was reduced in anti-SDF-1-injected eyes of rd10 mice (n = 8). *P < 0.05, **P < 0.01. Scale bars = 100 μm.