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Glut4 and Glut1 mislocalization in Tsc2−/− fibroblasts. A pair of isogenic Tsc2−/− and Tsc2+/+ embryonic fibroblast lines were grown in the absence or presence of FBS, fixed in 4% paraformaldehyde, and processed for immunofluorescence analyses of Glut4 and Glut1. Right column shows magnified views of boxed areas. Bar is equivalent to 20 μm.
