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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Am J Physiol Gastrointest Liver Physiol. 2008 Feb 21;294(4):G1009–G1016. doi: 10.1152/ajpgi.00017.2008.

Fig. 2.

Fig. 2

Expression of cholesterol 7α-hydroxylase (Cyp7a1) mRNA at early and midlactation. Real-time PCR was performed in duplicate on cDNA synthesized from liver total RNA from control female rats (open bars) and PP rats (shaded bars) at 4, 10, 16, and 22 h for n = 3–6 animals per group. Each bar represents the mean ± SD. A: there were no differences between control and PP day 3 rats. B: expression of Cyp7a1 mRNA was increased at 16 h in PP day 10 rats. *Significant increase in Cyp7a1 mRNA expression at 16 h in PP day 10 vs. control group (P < 0.001); significant interaction of the data (P < 0.0006) with a significant effect of time (P < 0.0001) and lactation (P < 0.03) was determined by 2-way ANOVA and Bonferroni post hoc test.