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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Am J Physiol Gastrointest Liver Physiol. 2008 Feb 21;294(4):G1009–G1016. doi: 10.1152/ajpgi.00017.2008.

Fig. 4.

Fig. 4

Expression of sterol 27-hydroxylase (Cyp27a1) mRNA during early and midlactation. Real-time PCR was performed in duplicate on cDNA synthesized from liver total RNA from control (open bars) or PP rats (shaded bars) at 4, 10, 16, or 22 h, n = 5–8 animals per group. Each bar represents the mean ± SD. A: Cyp27a1 was not different between control and PP day 3 rats, determined by two-way ANOVA. B: Cyp27a1 mRNA expression was decreased in PP day 10 rats. P < 0.03 effect of lactation determined by two-way ANOVA. No significant difference was found between control and the lactating animals at the time points measured (Bonferroni post hoc test).