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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Am J Physiol Gastrointest Liver Physiol. 2008 Feb 21;294(4):G1009–G1016. doi: 10.1152/ajpgi.00017.2008.

Fig. 6.

Fig. 6

Expression of sterol 12α-hydroxylase (Cyp8b1) mRNA and protein in control and midlactation rats. A: real-time PCR was performed in duplicate on cDNA synthesized from liver total RNA from control female rats (open bars) and PP day 10 rats (shaded bars) at 4, 10, 16, and 22 h. There were no differences between control and PP day 10 rats. B: Western blot analysis of Cyp8b1 protein expression in control and PP day 10 rats. Densitometric analysis of Cyp8b1 Western blots of Cyp8b1 density (INT/mm2). Each bar represents the mean ± SD of n = 3–8 (A) or n = 4 (B) animals per group. There were no significant differences found between control and PP day 10 rats. For Western blot analysis, Ponceau red staining was performed for each membrane to ensure equal loading in each lane.