Table 1. Primers used for real-time PCR.
18S forward | 5′-GTA ACC CGT TGA ACC CCA TT-3′ |
18S reverse | 5′-CCA TCC AAT CGG TAG TAG CG-3′ |
Cyp7a1 forward | 5′-TGC CTT CTG TTA CCG AGT GAT GTT-3′ |
Cyp7a1 reverse | 5′-ACC GGC AGG TCA TTC AGT TGC ACT-3′ |
Cyp27a1 forward | 5′-ATG TGG CCA ATC TTC TCT ACC-3′ |
Cyp27a1 reverse | 5′-GGG AAG GAA AGT GAC ATA GAC-3′ |
Cyp8b1 forward | 5′-GGC TGG CTT CCT GAG CTT ATT′3′ |
Cyp8b1 reverse | 5′-ACT TCC TGA ACA GCT CAT CGG-3′ |
SHP forward | 5′-CTT GCT AGA GGA ACC CAA CAG TGG T-3′ |
SHP reverse | 5′-AAC ACT GTA TGC AAA CCG AGG A-3′ |
Cyp7a1, cholesterol 7α-hydroxylase; Cyp27a1, sterol 27-hydroxylase; Cyp8b1, sterol 12α-hydroxylase; SHP, small heterodimer partner.