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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Am J Physiol Gastrointest Liver Physiol. 2008 Feb 21;294(4):G1009–G1016. doi: 10.1152/ajpgi.00017.2008.

Table 1. Primers used for real-time PCR.

18S forward 5′-GTA ACC CGT TGA ACC CCA TT-3′
18S reverse 5′-CCA TCC AAT CGG TAG TAG CG-3′
Cyp7a1 forward 5′-TGC CTT CTG TTA CCG AGT GAT GTT-3′
Cyp7a1 reverse 5′-ACC GGC AGG TCA TTC AGT TGC ACT-3′
Cyp27a1 forward 5′-ATG TGG CCA ATC TTC TCT ACC-3′
Cyp27a1 reverse 5′-GGG AAG GAA AGT GAC ATA GAC-3′
Cyp8b1 forward 5′-GGC TGG CTT CCT GAG CTT ATT′3′
Cyp8b1 reverse 5′-ACT TCC TGA ACA GCT CAT CGG-3′
SHP forward 5′-CTT GCT AGA GGA ACC CAA CAG TGG T-3′
SHP reverse 5′-AAC ACT GTA TGC AAA CCG AGG A-3′

Cyp7a1, cholesterol 7α-hydroxylase; Cyp27a1, sterol 27-hydroxylase; Cyp8b1, sterol 12α-hydroxylase; SHP, small heterodimer partner.