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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Dev Biol. 2008 Jan 22;315(1):114–124. doi: 10.1016/j.ydbio.2007.12.014

Figure 5.

Figure 5

Aggrecan protein and message are enriched in cells expressing glial markers. A, B, E8 chick midbrain cross-sections double-labeled for aggrecan immunoreactivity (brown) and nestin/NES and GLAST mRNAs (blue). Arrows point to cells that both contain intracellular aggrecan core protein and express the glial marker transcripts. C, Confocal XY projections of E9 hindbrain cross-sections processed for fluorescent in situ hybridization (FISH) for aggrecan/AGC mRNA (green) and for immunohistological localization of the glial marker BLBP (red). D, Confocal XY projections of FISH for aggrecan/AGC mRNA (green) and immunostaining for the astrocyte marker GFAP (red) from E13 midbrain cross-sections. Confocal overlay projections identify cells expressing aggrecan and the radial glial marker or the astrocyte marker (arrowheads). ZY projections of the merged pictures through the marked axes (white lines) are shown in the left side panels.