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. Author manuscript; available in PMC: 2009 Mar 15.
Published in final edited form as: Arch Biochem Biophys. 2008 Jan 11;471(2):134–145. doi: 10.1016/j.abb.2008.01.001

Figure 1.

Figure 1

Effect of GSH on O-debenzylation of BFC and BQ by CYP3A4 in Baculosomes. Panel a shows the dependence of the rate of oxidation of BFC on the concentration of this substrate at no glutathione added (circles, solid line) and in the presence of 1 mM (inverted triangles, long dash), 2 mM (squares, medium dash), 4 mM (diamonds, short dash), and 8 mM (triangles, dash-dot) GSH. The lines represent the result of fitting of the data sets to the Hill equation. Panel b shows the dependencies of the Hill coefficient on the glutathione concentration for O-debenzylation of BFC (closed circles, solid line) and BQ (open circles dashed line). The points shown on this plot represent the averages of from two to three individual measurements. The lines on this plot show the results of the fitting of these data sets to a hyperbolic (Michaelis-Menten) equation with KDapp values of 0.5 ± 0.2 and 0.24 ± 0.2 mM, respectively.