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. Author manuscript; available in PMC: 2009 Mar 15.
Published in final edited form as: Arch Biochem Biophys. 2008 Jan 11;471(2):134–145. doi: 10.1016/j.abb.2008.01.001

Figure 4.

Figure 4

Titration of CYP3A4 with 1-PB in the presence of 10 mM GSH. Panel a shows a series of spectra of 1.7 µM CYP3A4 in 0.1 M Na-Hepes buffer, pH 7.4, (25 °C) containing 10 mM GSH at no substrate added and in the presence of 0.8, 2.2, 4.5, 10, 21 and 43 µM 1-PB. The inset shows the difference spectra obtained by subtraction of the first spectrum of the series (no 1-PB added) from all subsequent spectra. Panel b illustrates the 1-PB-induced changes in the concentrations of water-coordinated low spin (circles), high-spin (inverted triangles), and the thiol(ate)-corrdinate state (squares) of the heme protein. The diamonds represent the total concentration of the enzyme.