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. Author manuscript; available in PMC: 2009 Mar 15.
Published in final edited form as: Arch Biochem Biophys. 2008 Jan 11;471(2):134–145. doi: 10.1016/j.abb.2008.01.001

Figure 6.

Figure 6

Interaction of CYP3A4(C58,C64)-BADAN with GSH monitored by the changes in the fluorescence of the probe. (a) A series of emission spectra of 1.5 µM enzyme recorded at 0, 0.17, 0.33, 0.66, 1.0, 1.5, and 2.6 mM GSH. (b) Relative fluorescence intensity versus GSH concentration. The line shows the fitting of the data sets to a hyperbolic (Michaelis-Menten) equation with KDapp = 1.6 mM and the maximal amplitude of the fluorescence decrease of 17%.