Table 2.
Effects of GSH and ANF on O-debenzylation of 7-BFC in enriched Baculosomes and human liver microsomes*.
| System | [ANF], µM | [GSH], mM | kcat, min−1 | S50, µM | N |
|---|---|---|---|---|---|
| Enriched Baculosomesa | 0 | 0 | 0.65 ±0.09 | 30.6 ±1.2 | 1.47 ±0.13 |
| 0 | 0.5 | 0.64 ±0.05 (0.909) | 14.6 ±2.8 (0.013) | 0.96 ±0.06 (0.022) | |
| 0 | 1 | 0.72 ±0.23 (0.668) | 36.9 ±2.6 (0.048) | 1.06 ±0.19 (0.071) | |
| 0 | 4 | 1.32 ±0.19 (0.014) | 47.8 ±5.6 (0.011) | 1.19 ±0.42 (6.10−4) | |
| 25 | 0 | 0.68 ±0.03 (0.583) | 5.82 ±0.17 (6.10−4) | 0.98 ±0.03 (0.019) | |
| 25 | 4 | 0.91 ±0.24 (0.323) | 16.7 ±3.0 (0.030) | 1.0 ±0.08 (0.011) | |
| Human liver microsomesb | 0 | 0 | 0.42 ±0.03 | 59.4 ±17.4 | 2.00 ±0.34 |
| 0 | 0.5 | 0.61 ±0.09 (0.011) | 64.8 ±17.5 (0.680) | 1.17 ±0.20 (0.040) | |
| 0 | 1 | 0.47 ±0.07 (0.325) | 56.0 ±11.3 (0.762) | 1.24 ±0.05 (0.044) | |
| 0 | 2 | 0.46 ±0.04 (0.253) | 58.4 ±18.6 (0.951) | 0.96 ±0.09 (0.063) | |
| 0 | 4 | 0.54 ±0.10 (0.065) | 116 ±42 (0.041) | 1.00 ±0.02 (0.027) | |
| 0 | 8 | 0.40 ±0.09 (0.490) | 200 ±47 (0.002) | 1.13 ±0.34 (0.072) | |
| 25 | 0 | 0.54 ±0.18 (0.220) | 16.7 ±1.1 (0.001) | 1.34 ±0.36 (0.190) | |
| 25 | 1 | 0.69 ±0.04 (1.10−4) | 23.2 ±9.0 (0.023) | 1.23 ±0.22 (0.092) | |
*
The values given in the Table were obtained by averaging the result of 2–4 individual measurements and the “±” values show the confidence interval calculated for p = 0.05. The values in parentheses represent the p-values of Student’s T-test for the hypothesis of equality of the respective values with that observed at no effector (GSH and/or ANF) added.
a
Enriched preparations were prepared using the Baculosomes lot 283776A.
b
In the case of human liver microsomes kcat values are apparent and calculated per total cytochrome P450 content.