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. 2008 Jun 11;3(6):e2387. doi: 10.1371/journal.pone.0002387

Figure 1. Mechanism proposed for the GFP folding interference assay using C-terminal GFP-based folding reporters.

Figure 1

(A) Fluorescence of GFP reflects correct folding of the test protein attached to the reporter. (B) Misfolded fusion proteins interfere with the folding of the downstream GFP domain rendering it non-fluorescent. (C) Internal translation sites or proteolysis can give rise to soluble, truncated polypeptides with new N-termini (N′, after scissors) that do not interfere with GFP folding. (D) Truncation artifacts not fused to the GFP escape detection.