Skip to main content
. 2008 Jun 11;3(6):e2387. doi: 10.1371/journal.pone.0002387

Figure 3. GFP insertion constructs.

Figure 3

(A) GFP insertion constructs (GFPi) contain NdeI and BamHI cloning sites for accepting test proteins (X) between the C and N-termini of a circular permutant of GFP. Flexible amino acid linkers L1 and L2 GGGSGGGS separate the protein of interest from the two GFP domains, (GFP1 and GFP2). GFPi 9/8 starts at amino-acid position 173 (beginning of beta strand 9) and ends at amino-acid position 172 (end of beta strand 8) of GFP (top). GFP insertion 8/7, starts at amino-acid 157 (beginning of strand 8), and ends at amino-acid 156 (end of strand 7) (bottom). (B) Chimeric GFP insertion constructs of folding reporter GFP (light gray) and superfolder GFP (dark gray) scaffoldings (FR = folding reporter GFP, SF = superfolder GFP).