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. 2008 Mar 12;3(3):e1762. doi: 10.1371/journal.pone.0001762

Figure 1. Alternating pattern of DHPR immuno-fluorescence signals and Second Harmonic Generation signals using multiphoton excitation in unfixed single wt fibres.

Figure 1

A, overlay of DHPR immuno-fluorescence (green) and the SHG signal (blue) simultaneously recorded in a representative wt fibre excited with a pulsed Ti:Sa laser at 880 nm. The signal intensity profile plot (B) confirms the tubular origin of the DHPR signal that alternates with the SHG-signal arising from the myosin within the A-bands.