Table 1.
fold enrich.b | ||||||||
spot ID# | func. cat.a | protein identity | Genbank ID | fibre | non-fibre | p-valuec | Mowse score | pep. countd |
2 | C&E | aconitate hydratase | 4586021 | 1.5 | 0.14 | 64 | 2 | |
3 | C&E | aconitate hydratase | 4586021 | 1.5 | 0.08 | 68 | 2 | |
17 | C&E | β-amylase | 1771782 | 8.8 | < 0.01 | 46 | 2 | |
39 | C&E | ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | 168312 | 1.5 | 0.25 | 85 | 2 | |
40 | C&E | ribulose-1,5-bisphosphate carboxylase, large subunit | 168312 | 2.0e | 0.08 | 180 | 4 | |
44 | C&E | ribulose-1,5-bisphosphate carboxylase, large subunit | 1834444 | 6.1 | < 0.01 | 129 | 5 | |
45 | C&E | ribulose-1,5-bisphosphate carboxylase, large subunit | 2687483 | 5.4 | < 0.01 | 130 | 4 | |
46 | C&E | ribulose-1,5-bisphosphate carboxylase, large subunit | 6983900 | 2.9 | < 0.01 | 232 | 6 | |
47 | C&E | ribulose-1,5-bisphosphate carboxylase, large subunit | 1817560 | 3.3 | < 0.01 | 250 | 5 | |
48 | C&E | enolase | 9581744 | 1.1 | 0.65 | 265 | 7 | |
49 | C&E | enolase | 8919731 | 1.1 | 0.93 | 158 | 3 | |
50 | C&E | enolase | 9581744 | 3.4 | 0.02 | 206 | 6 | |
51 | C&E | ribulose-1,5-bisphosphate carboxylase, large subunit | 4098530 | 2.8 | 0.04 | 103 | 4 | |
57 | C&E | fumarate hydratase | 108708038 | 2.5 | 0.01 | 83 | 2 | |
58 | C&E | fumarate hydratase | 15226618 | 1.6 | 0.33 | 100 | 4 | |
59 | C&E | 6-phosphogluconate dehydrogenase | 2529229 | 1.5 | 0.19 | 100 | 3 | |
63 | C&E | citrate synthase | 11066954 | 3.7 | < 0.01 | 123 | 4 | |
68 | C&E | phosphoglycerate kinase | 1161600 | 1.2 | 0.56 | 257 | 4 | |
71 | C&E | phosphoglycerate kinase | 92872324 | 1.7 | 0.06 | 426 | 7 | |
72 | C&E | ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | 66735801 | 96 | 3 | |||
73 | C&E | rubisco activase | 13430332 | 6.1 | < 0.01 | 70 | 3 | |
74 | C&E | rubisco activase | 170129 | 5.2 | < 0.01 | 61 | 3 | |
75 | C&E | phosphoglycerate kinase | 3328122 | 2.9 | 0.02 | 250 | 6 | |
77 | C&E | fructose-bisphosphate aldolase | 15227981 | 1.1 | 0.82 | 155 | 3 | |
78 | C&E | fructose-bisphosphate aldolase | 20204 | 2.4 | 0.03 | 102 | 2 | |
79 | C&E | fructose-bisphosphate aldolase | 15227981 | 1.1 | 0.6 | 116 | 2 | |
80 | C&E | fructose-bisphosphate aldolase | 20204 | 1.3 | 0.04 | 177 | 3 | |
81 | C&E | rubisco activase | 4261547 | 2.2 | 0.03 | 60 | 2 | |
82 | C&E | succinate-CoA ligase | 15225353 | 2.3 | 0.02 | 253 | 5 | |
83 | C&E | glyceraldehyde-3-phosphate dehydrogenase | 120666 | 2.6 | 0.01 | 76 | 2 | |
86 | C&E | glyceraldehyde-3-phosphate dehydrogenase | 3023813 | 1.1 | 0.49 | 71 | 3 | |
87 | C&E | glyceraldehyde-3-phosphate dehydrogenase | 74419004 | 3.8 | < 0.01 | 215 | 6 | |
90 | C&E | malate dehydrogenase | 18297 | 1.6 | 0.17 | 241 | 4 | |
91 | C&E | malate dehydrogenase | 18297 | 1.4 | 0.26 | 138 | 4 | |
92 | C&E | malate dehydrogenase | 10334493 | 3.3 | < 0.01 | 296 | 7 | |
93 | C&E | fructokinase | 31652274 | 6.7 | < 0.01 | 142 | 5 | |
94 | C&E | fructokinase | 31652274 | 2.2 | < 0.01 | 154 | 3 | |
96 | C&E | kinase/ribokinase, potential fructokinase | 15224669 | 2 | 0.01 | 208 | 8 | |
1 | ATP | AAA-ATPase | 86212372 | 1.6 | 0.24 | 322 | 10 | |
7 | ATP | ATPase, transitional endoplasmic reticulum | 7378614 | 1.2e | 0.65 | 101 | 4 | |
24 | ATP | vacuolar proton-ATPase | 50251203 | 2.6 | 0.02 | 585 | 13 | |
31 | ATP | ATP binding | 15221770 | 1 | 0.87 | 100 | 4 | |
35 | ATP | F1 ATPase | 12986 | 1.6 | 0.05 | 143 | 6 | |
40 | ATP | ATP synthase β subunit | 21684923 | 2.0e | 0.08 | 192 | 4 | |
42 | ATP | ATP synthase β subunit | 19685 | 1 | 0.99 | 675 | 12 | |
43 | ATP | ATP synthase β subunit | 56784991 | 1.8 | 0.06 | 307 | 7 | |
99 | ATP | F1-ATPase gammma subunit | 303626 | 1 | 0.66 | 84 | 3 | |
105 | ATP | vacuolar V-H+ATPase subunit E | 5733660 | 1.8 | 0.01 | 53 | 2 | |
106 | ATP | vacuolar V-H+ATPase subunit E | 5733660 | 1.1 | 0.82 | 100 | 4 | |
12 | CWP | β-galactosidase | 115437888 | 8.4 | < 0.01 | 43 | 3 | |
13 | CWP | β-galactosidase | 3641863 | 8.9 | < 0.01 | 42 | 2 | |
14 | CWP | β-galactosidase | 3641863 | 5.4 | < 0.01 | 105 | 5 | |
15 | CWP | β-galactosidase | 3641863 | 8.8 | < 0.01 | 96 | 5 | |
16 | CWP | β-galactosidase | 34913072 | 9.3 | < 0.01 | 72 | 4 | |
18 | CWP | MUCILAGE-MODIFIED 4 | 42562732 | 4.1 | < 0.01 | 57 | 2 | |
19 | CWP | rhamnose biosynthetic enzyme | 108707484 | 6.6 | < 0.01 | 100 | 6 | |
27 | CWP | phosphoglucomutase | 12585309 | 1.8 | 0.15 | 170 | 4 | |
28 | CWP | phosphoglucomutase | 6272281 | 3.7 | 0.02 | 122 | 5 | |
36 | CWP | UDP-glucose pyrophosphorylase | 6136112 | 1.4 | 0.37 | 82 | 3 | |
38 | CWP | UDP-glucose pyrophosphorylase | 82659609 | 3.5 | 0.01 | 166 | 6 | |
41 | CWP | UDP-glucose pyrophosphorylase | 9280626 | 1.6e | 0.1 | 129 | 6 | |
64 | CWP | β-galactosidase | 3641863 | 1.2 | 0.51 | 72 | 2 | |
76 | CWP | NAD-dependent epimerase/dehydratase (UXS6) | 15226950 | 6.1 | < 0.01 | 109 | 4 | |
88 | CWP | UDP-glucose 4-epimerase | 12643850 | 1.1 | 0.84 | 60 | 2 | |
101 | CWP | GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase | 12324315 | 2.3 | < 0.01 | 155 | 3 | |
104 | CWP | dTDP-D-glucose 4,6-dehydratase-like | 50253123 | 3 | < 0.01 | 56 | 2 | |
9 | 1C | Met synthase | 77556633 | 2 | < 0.01 | 222 | 6 | |
10 | 1C | Met synthase | 8439545 | 2.2 | < 0.01 | 105 | 3 | |
41 | 1C | S-adenosyl-L-homocysteine hydrolase | 1710838 | 1.6e | 0.1 | 174 | 5 | |
53 | 1C | serine hydroxymethyltransferase | 11762130 | 2.2 | 0.02 | 129 | 4 | |
60 | 1C | Met adenosyltransferase | 37051117 | 2.1 | 0.02 | 94 | 4 | |
55 | MemT | GDP dissociation inhibitor | 8439465 | 2 | 0.13 | 212 | 5 | |
56 | MemT | GDP dissociation inhibitor | 8439465 | 1.9 | 0.08 | 158 | 4 | |
95 | MemT | K+ channel β-subunit | 15219795 | 8.6 | 0.01 | 132 | 4 | |
102 | MemT | 34 kDa outer mitochondrial membrane porin-like protein | 83283993 | 1.7 | 55 | 2 | ||
103 | MemT | 36kDa porin I | 515358 | 3.9 | < 0.01 | 104 | 4 | |
5 | C&S | myosin heavy chain | 108710464 | 2.5 | 0.05 | 46 | 2 | |
6 | C&S | myosin heavy chain | T00727 | 3.6 | 0.01 | 48 | 2 | |
22 | C&S | dynamin central region | 92891191 | 3.1 | 0.09 | 83 | 3 | |
25 | C&S | dynamin-like | 21593776 | 1 | 0.77 | 143 | 4 | |
37 | C&S | β-tubulin | 295851 | 1.8 | 0.06 | 161 | 6 | |
52 | C&S | tubulin/FtsZ family, GTPase domain | 62734655 | 1.7 | 0.06 | 367 | 12 | |
69 | C&S | actin | 32186910 | 3.1 | 0.01 | 281 | 8 | |
70 | C&S | actin | 15242516 | 1.5 | 0.21 | 459 | 12 | |
4 | P&AA | elongation factor EF-2 | 6056373 | 2.5 | 0.02 | 40 | 3 | |
7 | P&AA | ClpC protease | 4105131 | 1.2e | 81 | 4 | ||
8 | P&AA | ClpC protease | 18423214 | 1.7 | 0.06 | 286 | 11 | |
11 | P&AA | HSP 90 | 1708314 | 1.7 | 0.01 | 312 | 10 | |
20 | P&AA | HSP 70-3 | 38325815 | 1.9 | 0.08 | 404 | 11 | |
21 | P&AA | HSP 70 | 62733235 | 1.7 | 0.11 | 612 | 13 | |
23 | P&AA | HSP 70 | 22636 | 2 | 0.04 | 100 | 3 | |
29 | P&AA | chaperonin CPN60-1 | 108706134 | 2.7 | 0.04 | 139 | 6 | |
30 | P&AA | chaperonin CPN60-1 | 108706134 | 1.5 | 0.04 | 327 | 7 | |
32 | P&AA | HSP 60 | 16221 | 2.1 | 0.02 | 140 | 4 | |
54 | P&AA | eukaryotic elongation factor 1A | 24371059 | 2.3 | 0.02 | 227 | 7 | |
61 | P&AA | 26S protease regulatory subunit | 1709798 | 2.1 | < 0.01 | 85 | 3 | |
62 | P&AA | translation initiation factor eIF-4A | 475221 | 1.6 | 0.09 | 262 | 9 | |
65 | P&AA | 26S proteasome subunit P45 | 92870338 | 1.9 | 0.11 | 90 | 3 | |
66 | P&AA | aminomethyltransferase | 3334196 | 3.7 | < 0.01 | 67 | 2 | |
67 | P&AA | elongation factor-1 alpha | 396134 | 1.2 | 0.5 | 54 | 3 | |
72 | P&AA | glutamine synthetase | 121341 | 1.7e | 0.26 | 119 | 4 | |
84 | P&AA | P0 ribosomal protein | 1143507 | 2.5 | < 0.01 | 155 | 3 | |
89 | P&AA | glutamate-ammonia ligase | 99698 | 1.2e | 0.5 | 65 | 3 | |
114 | P&AA | eukaryotic translation initiation factor 5A | 8778393 | 2 | 0.04 | 91 | 2 | |
26 | misc | nucleolar protein NOP5 | 108708132 | 1.4 | 0.37 | 47 | 2 | |
33 | misc | ferric leghemoglobin reductase | 5823556 | 1.6 | 0.2 | 124 | 4 | |
34 | misc | calreticulin | 3288109 | 1 | 0.9 | 78 | 3 | |
85 | misc | peroxidase | 1389835 | 2.4 | 0.03 | 214 | 7 | |
89 | misc | type IIIa membrane protein cp-wap13 | 2218152 | 58 | 3 | |||
97 | misc | annexin | 1429207 | 2.2 | 0.01 | 146 | 4 | |
98 | misc | annexin | 1429207 | 4.1 | 0.03 | 71 | 2 | |
100 | misc | enoyl-ACP reductase | 2204236 | 2.1 | 0.01 | 44 | 2 | |
107 | misc | protein kinase C inhibitor | 20062 | 2.8 | < 0.01 | 97 | 5 | |
108 | misc | 14-3-3 protein | 695767 | 2.7 | 0.01 | 44 | 3 | |
109 | misc | guanine nucleotide regulatory protein | 395072 | 1.5 | 0.31 | 64 | 2 | |
110 | misc | NAD(P)H dependent 6'-deoxychalcone synthase | 18728 | 1.1 | 0.82 | 56 | 3 | |
111 | misc | inorganic pyrophosphatase | 48927683 | 2.8 | 0.02 | 148 | 3 | |
112 | misc | maturase K | 33332553 | 3.4 | 0.01 | 55 | 2 | |
113 | misc | CBS (cystathionine β-synthase) domain-containing | 15238284 | 1.6 | 0.1 | 92 | 2 |
a) Functional category: ATPases (ATP); Cell wall polysaccharide metabolism (CWP); Cytoskeleton and secretion (C&S); Membrane transport (MemT); Miscellaneous (misc); One-carbon metabolism (1C); Primary carbon and energy metabolism (C&E); Protein and amino acid metabolism (P&AA). Only the highest scoring protein for each spot is categorized.
b) Fold enrichment in fibre tissues or non-fibre tissues as compared to the other tissue type, expressed as linear ratio of mean signal intensities.
c) P-value for a t-test of significant differences in mean signal intensities between fibre and non-fibre tissues.
d) Peptide count, i.e. the number of peptides per spot that match the Genbank ID shown.
e) Spots in which multiple proteins were identified. The intensity ratios shown may be due to differences in abundance of more than one protein.
Protein identities are sorted by functional category, in the order in which each category is presented in the text, and then alphabetically within each functional category. Additional data (including peptide sequences) is provided in Additional File 1.