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. 2008 Apr 30;8:52. doi: 10.1186/1471-2229-8-52

Table 1.

Protein identities based on peptide matches to Genbank protein databases

fold enrich.b
spot ID# func. cat.a protein identity Genbank ID fibre non-fibre p-valuec Mowse score pep. countd

2 C&E aconitate hydratase 4586021 1.5 0.14 64 2
3 C&E aconitate hydratase 4586021 1.5 0.08 68 2
17 C&E β-amylase 1771782 8.8 < 0.01 46 2
39 C&E ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit 168312 1.5 0.25 85 2
40 C&E ribulose-1,5-bisphosphate carboxylase, large subunit 168312 2.0e 0.08 180 4
44 C&E ribulose-1,5-bisphosphate carboxylase, large subunit 1834444 6.1 < 0.01 129 5
45 C&E ribulose-1,5-bisphosphate carboxylase, large subunit 2687483 5.4 < 0.01 130 4
46 C&E ribulose-1,5-bisphosphate carboxylase, large subunit 6983900 2.9 < 0.01 232 6
47 C&E ribulose-1,5-bisphosphate carboxylase, large subunit 1817560 3.3 < 0.01 250 5
48 C&E enolase 9581744 1.1 0.65 265 7
49 C&E enolase 8919731 1.1 0.93 158 3
50 C&E enolase 9581744 3.4 0.02 206 6
51 C&E ribulose-1,5-bisphosphate carboxylase, large subunit 4098530 2.8 0.04 103 4
57 C&E fumarate hydratase 108708038 2.5 0.01 83 2
58 C&E fumarate hydratase 15226618 1.6 0.33 100 4
59 C&E 6-phosphogluconate dehydrogenase 2529229 1.5 0.19 100 3
63 C&E citrate synthase 11066954 3.7 < 0.01 123 4
68 C&E phosphoglycerate kinase 1161600 1.2 0.56 257 4
71 C&E phosphoglycerate kinase 92872324 1.7 0.06 426 7
72 C&E ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit 66735801 96 3
73 C&E rubisco activase 13430332 6.1 < 0.01 70 3
74 C&E rubisco activase 170129 5.2 < 0.01 61 3
75 C&E phosphoglycerate kinase 3328122 2.9 0.02 250 6
77 C&E fructose-bisphosphate aldolase 15227981 1.1 0.82 155 3
78 C&E fructose-bisphosphate aldolase 20204 2.4 0.03 102 2
79 C&E fructose-bisphosphate aldolase 15227981 1.1 0.6 116 2
80 C&E fructose-bisphosphate aldolase 20204 1.3 0.04 177 3
81 C&E rubisco activase 4261547 2.2 0.03 60 2
82 C&E succinate-CoA ligase 15225353 2.3 0.02 253 5
83 C&E glyceraldehyde-3-phosphate dehydrogenase 120666 2.6 0.01 76 2
86 C&E glyceraldehyde-3-phosphate dehydrogenase 3023813 1.1 0.49 71 3
87 C&E glyceraldehyde-3-phosphate dehydrogenase 74419004 3.8 < 0.01 215 6
90 C&E malate dehydrogenase 18297 1.6 0.17 241 4
91 C&E malate dehydrogenase 18297 1.4 0.26 138 4
92 C&E malate dehydrogenase 10334493 3.3 < 0.01 296 7
93 C&E fructokinase 31652274 6.7 < 0.01 142 5
94 C&E fructokinase 31652274 2.2 < 0.01 154 3
96 C&E kinase/ribokinase, potential fructokinase 15224669 2 0.01 208 8
1 ATP AAA-ATPase 86212372 1.6 0.24 322 10
7 ATP ATPase, transitional endoplasmic reticulum 7378614 1.2e 0.65 101 4
24 ATP vacuolar proton-ATPase 50251203 2.6 0.02 585 13
31 ATP ATP binding 15221770 1 0.87 100 4
35 ATP F1 ATPase 12986 1.6 0.05 143 6
40 ATP ATP synthase β subunit 21684923 2.0e 0.08 192 4
42 ATP ATP synthase β subunit 19685 1 0.99 675 12
43 ATP ATP synthase β subunit 56784991 1.8 0.06 307 7
99 ATP F1-ATPase gammma subunit 303626 1 0.66 84 3
105 ATP vacuolar V-H+ATPase subunit E 5733660 1.8 0.01 53 2
106 ATP vacuolar V-H+ATPase subunit E 5733660 1.1 0.82 100 4
12 CWP β-galactosidase 115437888 8.4 < 0.01 43 3
13 CWP β-galactosidase 3641863 8.9 < 0.01 42 2
14 CWP β-galactosidase 3641863 5.4 < 0.01 105 5
15 CWP β-galactosidase 3641863 8.8 < 0.01 96 5
16 CWP β-galactosidase 34913072 9.3 < 0.01 72 4
18 CWP MUCILAGE-MODIFIED 4 42562732 4.1 < 0.01 57 2
19 CWP rhamnose biosynthetic enzyme 108707484 6.6 < 0.01 100 6
27 CWP phosphoglucomutase 12585309 1.8 0.15 170 4
28 CWP phosphoglucomutase 6272281 3.7 0.02 122 5
36 CWP UDP-glucose pyrophosphorylase 6136112 1.4 0.37 82 3
38 CWP UDP-glucose pyrophosphorylase 82659609 3.5 0.01 166 6
41 CWP UDP-glucose pyrophosphorylase 9280626 1.6e 0.1 129 6
64 CWP β-galactosidase 3641863 1.2 0.51 72 2
76 CWP NAD-dependent epimerase/dehydratase (UXS6) 15226950 6.1 < 0.01 109 4
88 CWP UDP-glucose 4-epimerase 12643850 1.1 0.84 60 2
101 CWP GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase 12324315 2.3 < 0.01 155 3
104 CWP dTDP-D-glucose 4,6-dehydratase-like 50253123 3 < 0.01 56 2
9 1C Met synthase 77556633 2 < 0.01 222 6
10 1C Met synthase 8439545 2.2 < 0.01 105 3
41 1C S-adenosyl-L-homocysteine hydrolase 1710838 1.6e 0.1 174 5
53 1C serine hydroxymethyltransferase 11762130 2.2 0.02 129 4
60 1C Met adenosyltransferase 37051117 2.1 0.02 94 4
55 MemT GDP dissociation inhibitor 8439465 2 0.13 212 5
56 MemT GDP dissociation inhibitor 8439465 1.9 0.08 158 4
95 MemT K+ channel β-subunit 15219795 8.6 0.01 132 4
102 MemT 34 kDa outer mitochondrial membrane porin-like protein 83283993 1.7 55 2
103 MemT 36kDa porin I 515358 3.9 < 0.01 104 4
5 C&S myosin heavy chain 108710464 2.5 0.05 46 2
6 C&S myosin heavy chain T00727 3.6 0.01 48 2
22 C&S dynamin central region 92891191 3.1 0.09 83 3
25 C&S dynamin-like 21593776 1 0.77 143 4
37 C&S β-tubulin 295851 1.8 0.06 161 6
52 C&S tubulin/FtsZ family, GTPase domain 62734655 1.7 0.06 367 12
69 C&S actin 32186910 3.1 0.01 281 8
70 C&S actin 15242516 1.5 0.21 459 12
4 P&AA elongation factor EF-2 6056373 2.5 0.02 40 3
7 P&AA ClpC protease 4105131 1.2e 81 4
8 P&AA ClpC protease 18423214 1.7 0.06 286 11
11 P&AA HSP 90 1708314 1.7 0.01 312 10
20 P&AA HSP 70-3 38325815 1.9 0.08 404 11
21 P&AA HSP 70 62733235 1.7 0.11 612 13
23 P&AA HSP 70 22636 2 0.04 100 3
29 P&AA chaperonin CPN60-1 108706134 2.7 0.04 139 6
30 P&AA chaperonin CPN60-1 108706134 1.5 0.04 327 7
32 P&AA HSP 60 16221 2.1 0.02 140 4
54 P&AA eukaryotic elongation factor 1A 24371059 2.3 0.02 227 7
61 P&AA 26S protease regulatory subunit 1709798 2.1 < 0.01 85 3
62 P&AA translation initiation factor eIF-4A 475221 1.6 0.09 262 9
65 P&AA 26S proteasome subunit P45 92870338 1.9 0.11 90 3
66 P&AA aminomethyltransferase 3334196 3.7 < 0.01 67 2
67 P&AA elongation factor-1 alpha 396134 1.2 0.5 54 3
72 P&AA glutamine synthetase 121341 1.7e 0.26 119 4
84 P&AA P0 ribosomal protein 1143507 2.5 < 0.01 155 3
89 P&AA glutamate-ammonia ligase 99698 1.2e 0.5 65 3
114 P&AA eukaryotic translation initiation factor 5A 8778393 2 0.04 91 2
26 misc nucleolar protein NOP5 108708132 1.4 0.37 47 2
33 misc ferric leghemoglobin reductase 5823556 1.6 0.2 124 4
34 misc calreticulin 3288109 1 0.9 78 3
85 misc peroxidase 1389835 2.4 0.03 214 7
89 misc type IIIa membrane protein cp-wap13 2218152 58 3
97 misc annexin 1429207 2.2 0.01 146 4
98 misc annexin 1429207 4.1 0.03 71 2
100 misc enoyl-ACP reductase 2204236 2.1 0.01 44 2
107 misc protein kinase C inhibitor 20062 2.8 < 0.01 97 5
108 misc 14-3-3 protein 695767 2.7 0.01 44 3
109 misc guanine nucleotide regulatory protein 395072 1.5 0.31 64 2
110 misc NAD(P)H dependent 6'-deoxychalcone synthase 18728 1.1 0.82 56 3
111 misc inorganic pyrophosphatase 48927683 2.8 0.02 148 3
112 misc maturase K 33332553 3.4 0.01 55 2
113 misc CBS (cystathionine β-synthase) domain-containing 15238284 1.6 0.1 92 2

a) Functional category: ATPases (ATP); Cell wall polysaccharide metabolism (CWP); Cytoskeleton and secretion (C&S); Membrane transport (MemT); Miscellaneous (misc); One-carbon metabolism (1C); Primary carbon and energy metabolism (C&E); Protein and amino acid metabolism (P&AA). Only the highest scoring protein for each spot is categorized.

b) Fold enrichment in fibre tissues or non-fibre tissues as compared to the other tissue type, expressed as linear ratio of mean signal intensities.

c) P-value for a t-test of significant differences in mean signal intensities between fibre and non-fibre tissues.

d) Peptide count, i.e. the number of peptides per spot that match the Genbank ID shown.

e) Spots in which multiple proteins were identified. The intensity ratios shown may be due to differences in abundance of more than one protein.

Protein identities are sorted by functional category, in the order in which each category is presented in the text, and then alphabetically within each functional category. Additional data (including peptide sequences) is provided in Additional File 1.