Figure 3.

Complementation construction and control experiments. (A) Schematic picture of the complementation construct. Schematic depiction of the construct used for the complementation of slr1649 with the cryptophytic orf222. The upper figure displays the wild type situation. The lower figure shows the insertion site of orf222, without its putative transit peptide and the aadA gene into slr1649 by simultaneously affecting the reading frame of slr1649 and its cis-acting upstream signals. (B) Immunoblot with Slr1649 (upper) and Slr1470 (lower) specific antibodies. Cells from Δslr1649, wild type and complemented strains were disrupted and the protein extracts were separated by SDS-PAGE. Neither in the fraction of Δslr1649 cells nor in the fraction of the complemented strain were signals of the Slr1649 antibody detectable. Specific polyclonal Slr1470 antibodies were used as a loading control and clear signasl at the expected size were obtained in all three protein fractions.