Table 2.
DDT sequencing of regions selected based on pilot SS14/Nichols comparison using microarray hybridization experiments
Region no. | ORFa | Size of sequenced region (nt) | Left coordinatea | Right coordinatea | Newly found changes | Confirmation of SNPs identified by CGSb |
1 | TP0017 | 848 | 18454 | 19301 | - | - |
2 | TP0070 | 339 | 75493 | 75831 | - | - |
3 | TP0094 | 1011 | 102879 | 103889 | - | - |
4 | TP0123 | 1083 | 143207 | 144289 | - | - |
5 | TP0192 | 748 | 206663 | 207410 | - | - |
6 | TP0200 | 264 | 210183 | 210446 | - | - |
7 | TP0291 | 834 | 304706 | 305539 | - | - |
8 | TP0319 | 1014 | 334847 | 335860 | - | 1 SNP |
9 | TP0321–TP0322 | 2640 | 336149 | 338788 | - | - |
10 | TP0323 | 851 | 338885 | 339735 | - | 1 SNP |
11 | TP0376 | 806 | 400903 | 401708 | - | - |
12 | TP0377 | 78 | 401851 | 401928 | - | - |
13 | TP0516 | 1533 | 556351 | 557883 | - | - |
14 | TP0519 | 1277 | 559215 | 560491 | - | - |
15 | TP0580 | 1242 | 630328 | 631569 | - | - |
16 | TP0587 | 183 | 639620 | 639802 | - | - |
17 | TP0633 | 776 | 691437 | 692212 | - | - |
18 | TP0683 | 1047 | 746899 | 747945 | - | - |
19 | TP0799–TP0800 | 2168 | 866136 | 868303 | - | - |
20 | TP0806 | 1397 | 875808 | 877204 | - | - |
21 | TP0807 | 165 | 877407 | 877571 | - | - |
22 | TP0808 | 187 | 877632 | 877818 | - | - |
23 | TP0877 | 998 | 953710 | 954707 | - | 2 SNPs |
24 | TP0933 | 2023 | 1013098 | 1015120 | - | - |
25 | TP0952 | 1438 | 1032341 | 1033778 | - | 1 SNP |
26 | TP0961 | 1216 | 1041973 | 1043188 | - | - |
27 | TP0980 | 975 | 1063047 | 1064021 | - | - |
ORF – open reading frame; nt – nucleotide; SNP – single nucleotide polymorphism; aas described in [3]; bSNPs identified using CGS in these regions were verified by DDT sequencing.