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Real time analysis of [CRP] determination using RAP using a sandwich immunoassay. The trace shows typical data for the initial injection of a CRP containing sample (39 ng/mL CRP). Signal is seen as association of the CRP onto the capture antibody (Direct Capture Assay; t = 300–600 s). Next, sheep anti-CRP is injected to give a Direct Sandwich Assay. Again, an increase in signal is seen as association (t = 1100 – 1400 s).
