Table 1.

Determination of serum CRP concentration using different RAP assay formats.

	Cardiovascular Risk (AHA/CDC guidelines)/[CRP] g/mL
	LOW <1	Ave 1 – 3	HIGH 3 – 5	V. HIGH > 10
Spiked Serum [CRP]	0.1	1.2	3.61	10.4	34.8	94.8
hsCRP ELISA	0.092	1.26	4.35	9.6	44	116
Direct Capture	3.728	4.53	7.56	11.9	41	113
Homogenous Sandwich	3.4	3.4	6.3	15.5	53	120
Direct Sandwich	0.14	1.45	3.3	9.9	45.5	114

Results are compared with that obtained by commercial hsCRP ELISA.

Cardiovascular risk is considered at serum levels > 3 μg/ml. Normal horse serum was independently spiked with human CRP, these samples were diluted from 1/100 to 1/6000 for assay. Sandwich assays using RAP employ 0.225 μg/mL Sheep anti-CRP.