Fig. 3.

DmWRNexo mutant flies are sensitive to camptothecin (CPT). Flies heterozygous for the CG7670^e04496 allele were crossed and progeny reared on medium supplemented with various concentrations of CPT. The proportion of all emerging adult flies that were homozygous for CG7670^e04496 was plotted against CPT concentration; heterozygous flies were fully viable at all CPT concentrations tested. The lower frequency of homozygous flies observed at increasing drug doses demonstrates hypersensitivity to CPT. n represents the ratio of CG7670^e04496 homozygous: heterozygous flies. p-values refer to the Fisher exact test for the probability that the proportion of homozygotes was less than that of the control value (0 µm CPT). The error bars show binomial 95% confidence intervals.