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. 2008 Jun;7(3):318–334. doi: 10.1111/j.1474-9726.2008.00380.x

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© The Authors Journal compilation © Blackwell Publishing Ltd/The Anatomical Society of Great Britain and Ireland 2008

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Fig. 1 — Age-related change in the number of proliferating cells in the adult midgut. (A) Age-related change in 5-bromodeoxyuridine (BrdU) incorporation levels in the adult midgut of wild-type flies. Two-, 30- and 50-day-old wild-type flies were fed on BrdU for 10 days, and stained with anti-BrdU. Overlay (DAPI, blue; anti-BrdU, red). Magnification is ×400. (B) Age-related change in the number of proliferating cells in the midgut. (a–d) The guts of 3-, 30-, 45- and 60-day-old wild-type flies were labeled with anti-phospho-histone H3 (arrow). Overlay (DAPI, blue; antiphospho-histone H3, red). Scale bar, 20 µm. (e) Relative number of the phospho-histone H3-positive cells detected per midgut of the 3-, 30-, 45- and 60-day-old wild-type flies. The number of phospho-histone H3-positive cells detected per midgut of 3-day-old flies was set at 1. Results are expressed as the mean ± SE values of 41–45 adults. Two asterisks represent P < 0.001 as compared to the 3-day-old flies.