(A) Gd-DTPA-K6Aβ1–30 is not toxic in cell culture
As determined by the MTT assay, Gd-DTPA-K6Aβ1–30 and K6Aβ1–30 had no effect on viability of human neuroblastoma cells (SK-N-SH). DTPA-K6Aβ1–30 was slightly neurotrophic, whereas Aβ1–42 was toxic as is well established. *, p<0.05 **, p<0.01; (One-Way Analysis of Variance, Neuman Keuls post hoc test versus control).
(B) Gd-DTPA-K6Aβ1–30 binds to Aβ1–42 with high affinity (KD=3.2 nM ± 1.2).