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A, Strategy for polymerase chain reaction genotyping of INT1 alleles by use of forward primers INT1U and 5det-INT1 and reverse primer 3det-INT1. B, Genomic DNA from wild-type BWP17 (lane 1), VBIDM1 (INT1/int1::ARG4; lane 2), and VBIDM2 (int1::ARG4/int1::URA3; lane 3).
