. Author manuscript; available in PMC: 2008 Jun 2.

Published in final edited form as: J Infect Dis. 2008 Apr 1;197(7):981–989. doi: 10.1086/529203

Table 4.

Supplementary primers used in this study.

Primer	Sequence, 5′-3′
Targeted gene disruption of INT1
INT1FKO	ATGAACTCAACTCCAAGTAAATTATTACCGATAGATAAACATTCTCATTTACAATT ACAGCCTGTTTTCCCAGTCACGACGTT
INT1RKO	TTGAAGCATCAAATTTAGCCATGGTTGACGTCTGAACCGATTTCTATAGATAATTT CTTGTAAATTGTGGAATTGTGAGCGGATA
Detection of targeted INT1
5Det-INT1	TTCTCCATCTATCCATTCCTC
3Det-INT1	CAAAATGGGCATATATTTGCC
INT1U	GTGCGGGTTCTAAACCAA
Construction of Pep₂₆₃ expression vectors
DDC11F	ATAGAATTCACGGATTAGAAGCCGCCGAGCGGGTGAGAGC
DDC11R	AGCAAGCTTGATATCGATCGCATGCTTCTTTTAACAATGGATC
DDC15F	ATAGCATGCACCACCATCACCATCACATGAACTCAACTCCAAGTAAATTATTACCG
DDC15R	AGCAAGCTTTCAGTGCTTTAATTTCATATCTGTCAATGCCTCTGGTTCCGAATTGAC
DDC13F	AGCAAGCTTGTTTAAACCCGCTGATCTGATAACAA
DDC1R	TAAGTCGACAATTCTCTTAGGATTCGATTCACATTC
DDC43F	CCCCGGATCCATTGTTAAAAGAAGCATGAACTCAACTCCAAGTAAATTATTACCG
DDC29R	CCCCAAGCTTTCAATGGTGATGGTGATGGTGCTTTAATTTCATATCTGTCAATGC
CUG to CTG mutagenesis
DCMUT2F	CAACGAAGTCAATTCGGAACCAGAG
DCMUT2R	CTCTGGTTCCGAATTGACTTCGTTG
Pep₂₆₃ COOH deletion mutants
DDC44F	CCCCGGATCCATTGTTAAAAGAAGCATGCACCACCATCACCATCACATGAACTCA
DDC33R	CCCCAAGCTTTCATTTCATATCTGTCAATGCCTCTGGTTCCGAATTGACTTC
DDC34R	CCCCAAGCTTTCATAATTTCATTGTCAATGCCTCTGGTTCCGAATTGAC
DDC35R	CCCCAAGCTTTCACTTTAATTTCATATCTGTCAATGCCTCTGGTTCCGAATT
Amino acid 263 substitution
DDC43F	CCCCGGATCCATTGTTAAAAGAAGCATGAACTCAACTCCAAGTAAATTATTACCG
DDC36R	CCCCAAGCTTTCAATGGTGATGGTGATGGTGCGCCTTTAATTTCATATCTGTCAA
DDC37R	CCCCAAGCTTTCAATGGTGATGGTGATGGTGGCGCTTTAATTTCATACTTGTCAA
DDC40R	CCCCAAGCTTTCAATGGTGATGGTGATGGTGATCCTTTAATTTCATACTTGTCAA
Sequencing
T3	AATTAACCCTCACTAAAGGG
T7	GTAATACGACTCACTATAGGGC

NOTE. Mutated codons are in bold font.