Table 1.
Sequences and Product Sizes of PCR Primers
| Gene | Primer | Sequence | Cycles/T° C* | Produt Size(bp) |
|---|---|---|---|---|
| IL-6 | Forward
Reverse |
CTCCTTCTCCACAAGCGCCTTC
GCGCAGAATGAGATGAGTTGTC |
24/55 | 583 |
| IL-8 | Forward
Reverse |
GCAGTTTTGCCAAGGAGTGCT
GCATCTGGCAACCCTACAACA |
30/55 | 347 |
| TNF-α | Forward
Reverse |
GAAAGCATGATCCGGGACGTG
GATGGCAGAGAGGAGGTTGAC |
26/60 | 510 |
| iNOS | Forward
Reverse |
ACCTTCAAGGCAGCCTGTGAGACGT
AGTAGAACCTGGGCTTCAGAATGGG |
35/55 | 700 |
| Mn-SOD | Forward
Reverse |
GAGTTGCTGGAAGCCATCAAACGT
GTATCTTTCAGTTACATTCTCCCA |
26/55 | 306 |
| hBD-2 | Forward
Reverse |
CCAGCCATCAGCCATGAGGGT
GGAGCCCTTTCTGAATCCGCA |
35/62 | 255 |
| LL-37 | Reverse
Forward |
GTCCCCATACACCGCTTCAC
ATCATTGCCCAGGTCCTCAG |
40/62 | 251 |
| GAPDH | Forward
Reverse |
CACCACCAACTGCTTAGCAC
CCCTGTTGCTGTAGCCAAAT |
30/55 | 515 |