Figure 2.

Hypoxia regulates the expression of CTGF mRNA in human trophoblasts. PHT cells from term normal placentas were cultured in standard culture conditions (FiO₂ = 20%) or in two conditions of reduced FiO₂, 8% or less than or equal to 1%. CTGF transcript expression was determined using RT-qPCR as described in Materials and Methods. A, Time course for the effect of FiO₂ less than or equal to 1%, depicting the fold change in CTGF expression between the two conditions (*, P < 0.05, Mann-Whitney U test; n = 5). B, The influence of oxygen concentration on the expression of CTGF transcript (*P < 0.05, Kruskal-Wallis one-way ANOVA with post hoc Dunnett’s test; n = 3).