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. 2008 Feb 28;149(6):3025–3036. doi: 10.1210/en.2007-1129

Figure 4.

Figure 4

Stimulation of Rgc32 expression by hCG in rat granulosa cells in vitro. Autoradiograph of a representative Northern blot analysis shows Rgc32 mRNA and ribosomal protein L32 mRNA in granulosa cells obtained from rat preovulatory ovaries (48 h after PMSG) and cultured in medium alone (control), or with hCG (1 IU/ml) for 0, 3, 6, 12, or 24 h. Relative levels of Rgc32 mRNA were normalized to the L32 band in each sample (mean ± sem; n = 4 independent culture experiments). Bars with no common superscripts are significantly different (P < 0.05).